Request Molecular Genetics Core (MGC) services though MiCORES.
The Molecular Genetics Core (MGC) provides services related to the application of genetic methodologies to diabetes-related research.
The MGC focuses predominantly on the generation of targeted genetic rodent models, using CRISPR/Cas9 system in mouse or rat embryos:
- Gene edited alleles
- Knockout alleles
- loxP-flanked alleles
- Large DNA fragment knock-in alleles (gene insertions, e.g., Cre and Flp recombinases, fluorescent reporters (e.g., GFP)
- Cost includes targeting design, testing of guide RNAs for DNA targeting, and screening of potential founders by PCR and sequence analysis. Note: cost does NOT include injection costs billed by the UM Transgenic Animal Model Core
- Application of CRISPR-Cas9 technology in specific genetic backgrounds is available on request, at extra cost billed by the UM Transgenic Animal Model Core.
Generation of specialty viral vectors:
- Constitutive or cre-inducible AAVs expressing transgenes of interest
- Viral production costs are paid directly to the Viral Vector Core by user. ALL costs may be waived for reagents likely to be of general interest, providing that PI is willing to allow distribution to other MDRC members following initial testing.
Specialty viral reagents:
- Defective pseudotyped rabies viruses for tract-tracing, including AAV helper viruses
- Under development: AAV-driven CRISPR/Cas9 reagents for gene ablation in animals; yellow fever virus for anterograde neuronal circuit tracing.
Cost of Services
Rates Effective 3/7/2023 - 03/01/2024
* non-UM customers please contact David Olson for quote
Rates without Transgenic Costs
Knock-In Rate (large DNA fragment; loxP-flanked alleles)
$7,431.95 per test
$5,675.36 per test
$2,724.51 per test
Lab Tech Services
$32 per hour
Total costs based on current Transgenic Animal Model Core rates (October 2023):
* External Customers, please download, complete, and return the signed Service Agreement, along with the Service Request form and return to David Olson.
David P. Olson, MD, PhD
North Campus Research Complex (NCRC), Building 25-3785
Remember to cite the Michigan Diabetes Research Center (MDRC) in publications as follows:
“The project described was supported by Grant Number P30DK020572 (MDRC) from the National Institute of Diabetes and Digestive and Kidney Diseases”